IMAGEJ SOFTWARE WESTERN BLOT FOR FREE
IMAGEJ SOFTWARE WESTERN BLOT MAC OS
IMAGEJ SOFTWARE WESTERN BLOT WINDOWS

ImageJ will open a new window that has windows stacked horizontally, with one window for each of your lanes.

After you have dragged the last rectangle over the last lane, indicate that you are finished by selecting: Analyze > Gels > Plot lanes (or command -3).

(Note: You don't have to be careful about the height of the boxes, as ImageJ will automatically align the boxes.)

Repeat until all the lanes have been highlighted with a rectangle.

Indicate this is another lane by selecting: Analyze > Gels > Select next lanes (or command -2)

Using the mouse, click inside the rectange and drag (a second box will drag) to the second lane.

Indicate that this is the first lane of the gel by selecting: Analyze > Gels > Select First lane (or command - 1) If there are multiple bands in the lane, make sure there is white space between the bands. Include some white space above and below the band (or bands).

Use the rectangle tool to create a vertical rectangle in the first lane.

You have to do this again, because the powerpoint file saves it as an RGB tiff file.

Convert the image to 8-bit (Image > Type > 8-bit).

Open the TIFF file you created above in ImageJ.

Powerpoint saves a folder with the filename, and then saves each slide as slide1.tiff, etc.

Save the file as a TIFF file using Powerpoint save as command.

Do not change sizes or adjust the images in anyway.

Note: You can also label everything, put in ladder images and other things, as long as you leave white space around the images.

Line them up, leaving white space in between each one.

Open a Powerpoint file and drag all your image files into powerpoint.

Repeat this process using your loading control image file, if it is a different file.

Save the file, and repeat with the other file copies, if you have more than one set of bands on the same original image.

You should now have a very small file with a rectangle that only includes the bands you want to measure Try to draw the rectangle as small as possible around the horizontal line of bands.

Crop the image by highlighting the bands you want to measure using the Rectangle tool.

Enlarge the image using the Magnifying Glass tool so you mainly just see the bands you want to measure.

If there are more than one set of bands that you want to analyze, make as many copies of the file as there are bands that you want to analyze.

Select at least 6 gridlines to help you judge whether or not the bands are horizontal.

Select the angle you want (it will rotate clockwise).

Rotate the image so the bands are lined up horizontally (Image > Tranform > Rotate).

If you have black background and white bands, invert the image (Edit > Invert).

Convert the image to 8-bit (Image > Type > 8-bit).

Save the files as TIFF file using a different name than the original.

Some may prefer popular software such as Adobe Photoshop, which is the world’s best imaging and design app. To put it simply, this software is not everyone’s cup of tea. Although, you’ll have to spend your time studying some of its crucial features since it’s designed for a specific audience. ImageJ is simply the best image-processing freeware for scientific purposes.

IMAGEJ SOFTWARE WESTERN BLOT FOR FREE

You can access the download link for free on the software’s official website.

IMAGEJ SOFTWARE WESTERN BLOT MAC OS

Since the software is written in Java, you can run it on Windows, Mac OS X, and Linux in both 32-bit and 64-bit modes. While its nature as an online applet allows you to develop your own mini-programs. You can also create and debug macros to automate the tasks you used most often. The software also allows you to download or write additional plug-ins to customize the program to fit your research needs. You can also make your own image calculations using the program and create statistics according to your preferred parameters such as mean and standard deviations. It is simply interactive which allows you to easily edit and analyze images for printing. Basic functions of the toolbar includes a wide range of measurement tools, image selection and enhancement, geometric operations, scaling and color processing. The software’s mini structure allows you to display and process multiple images at the same time. Once you’ve downloaded the program, you’ll get a mini toolbar which gives you access to the program’s main functions including the menu bar and status/progress bar. The software is also a public domain, which means you’re allowed to add plug-ins and make changes to its program and share it to the user community without any copyright restriction. It was created and constantly revamped by US software developer Wayne Rasband at the National Institutes of Health. ImageJ is an open-source freeware designed for scientific image processing and analysis. After its release, the software became a significant aid for everyday scientific researches and discoveries.

Imagej software western blot